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M9480558.TXT
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1994-08-20
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Document 0558
DOCN M9480558
TI Antisense oligonucleotide complementary to endogenous retroviral MCF env
gene inhibits both BFU-E and CFU-S colony formation in mice.
DT 9410
AU Chernukhin IV; Khaldoyanidi SK; Dikovskaya DV; Svinarchuk FP; Vlasov VV;
Gaidul KV; Institute of Clinical Immunology, Novosibirsk, Russian;
Federation.
SO FEBS Lett. 1994 Jul 11;348(2):197-200. Unique Identifier : AIDSLINE
MED/94307424
AB A possible biologic activity of endogenously expressed env sequence of
retroviral mink cell focus-forming virus (MCF) genome for hematopoietic
colony formation was studied in mice. Antisense 20-mer complementary to
MCF env sequence was used to detect the result of blockage of this gene
translation on the potency of marrow cells to form colonies of erythroid
(BFU-E), myeloid granulocyte-macrophage (CFU-GM), and stem cell (day 11
CFU-S) hematopoietic compartments. A large relative decrease in BFU-E
number was found in bone marrow cell cultures preincubated with
antisense oligonucleotide during 4 h, whereas CFU-GM colonies remained
unaffected. A marked reduction of CFU-S colony formation was also
registered under antisense oligomer influence. Following a decreased
proliferation of erythroid progenitors, we suggest the mechanism by
which antisense oligonucleotide could cause the loss of colony
formation. Taken together, these data allow to propose that the
expression of this gene is naturally significant for hematopoietic
progenitor activity exerting some property of env gene products to
regulate the growth of erythroid and multilineage hematopoietic
precursors.
DE Amino Acid Sequence Animal Base Sequence Cell Division/DRUG EFFECTS
Cells, Cultured Colony-Forming Units Assay *Genes, env Hematopoietic
Stem Cells/CYTOLOGY/*DRUG EFFECTS Male Mice Mice, Inbred C57BL Mink
Cell Focus-Inducing Viruses/*GENETICS Molecular Sequence Data
Oligonucleotides, Antisense/GENETICS/*PHARMACOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).